In-Patient Evolution of a High-Persister Escherichia coli Strain With Reduced In Vivo Antibiotic Susceptibility

Authors

Joshua B Parsons
Ashelyn E Sidders
Amanda Z Velez
Blake M Hanson
Michelle Angeles-Solano
Felicia Ruffin
Sarah E Rowe
Cesar A Arias
Vance G Fowler
Joshua T Thaden
Brian P Conlon

Publication Date

1-16-2024

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abstract

Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Despite appropriate antibiotic treatment, relapse of GNB-BSI with the same bacterial strain is common and associated with poor clinical outcomes and high healthcare costs. The role of persister cells, which are sub-populations of bacteria that survive for prolonged periods in the presence of bactericidal antibiotics, in relapse of GNB-BSI is unclear. Using a cohort of patients with relapsed GNB-BSI, we aimed to determine how the pathogen evolves within the patient between the initial and subsequent episodes of GNB-BSI and how these changes impact persistence. Using

Keywords

Humans, Animals, Mice, Escherichia coli, Anti-Bacterial Agents, Bacteremia, Sepsis, Recurrence

Comments

Supplementary Materials

PMID: 38190524