Publication Date
3-1-2021
Journal
American Journal of Physiology-Endocrinology and Metabolism
DOI
10.1152/ajpendo.00472.2020
PMID
33427052
PMCID
PMC7988784
PubMedCentral® Posted Date
1-11-2021
PubMedCentral® Full Text Version
Post-print
Published Open-Access
no
Keywords
Animals, Arginine, Biological Availability, Citrulline, Female, Hydrolases, Kinetics, Male, Polyethylene Glycols, Swine, Tissue Distribution, arginine, arginine deiminase, citrulline, swine, tracer kinetics
Abstract
Pegylated arginine deiminase (ADI-PEG20) results in the depletion of arginine with the production of isomolar amounts of citrulline. This citrulline has the potential to be utilized by the citrulline recycling pathway regenerating arginine and sustaining tissue arginine availability. The goal of this research was to test the hypothesis that ADI-PEG20 depletes circulating arginine in pigs but maintains tissue arginine concentration and function, and to characterize the kinetics of citrulline and arginine. Two multitracer approaches (bolus dose and primed-continuous infusion) were used to investigate the metabolism of arginine and citrulline in Control (
Graphical Abstract
Included in
Animals Commons, Diseases Commons, Medical Sciences Commons, Medical Specialties Commons