Language
English
Publication Date
9-5-2023
Journal
Transfusion
DOI
10.1111/trf.17532
PMID
37668082
PMCID
PMC10655130
PubMedCentral® Posted Date
10-1-2024
PubMedCentral® Full Text Version
Author MSS
Abstract
INTRODUCTION: Warm antibody-mediated autoimmune hemolysis (WAIHA) is most often due to immunoglobulin G (IgG) antibodies and is detected by direct antiglobulin test (DAT). However, about 10% cases of hemolytic anemia are DAT negative. Herein, we describe a patient with DAT-negative hemolytic anemia due to an anti-IgA antibody. We investigate if isolated IgA can promote erythrophagocytosis.
METHODS: We isolated patient and control IgA on Jacalin agarose. Isolated IgA was used to sensitize healthy ABO/Rh-matched donor red blood cells (RBC). Antibody binding was examined by flowcytometry. The effect of IgA on erythrophagocytosis was evaluated using Macrophage colony stimulating factor 1 (M-CSF)-differentiated autologous macrophages by Giemsa staining and immunofluorescence microscopy.
RESULTS: We show that isolated IgA from the serum can bind to red cells. In addition, RBCs were phagocytosed efficiently by autologous macrophages in the presence of patient IgA.
CONCLUSION: Our results show that IgA antibodies are capable of inducing erythrophagocytosis like IgG antibodies in the absence of complement fixation.
Keywords
Humans, Anemia, Hemolytic, Autoimmune, Immunoglobulin A, Hemolysis, Autoantibodies, Erythrocytes, Immunoglobulin G, Lymphohistiocytosis, Hemophagocytic, Coombs Test, hemolysis, IgA antibodies, autoimmune, trogocytosis, phagocytosis
Published Open-Access
yes
Recommended Citation
Jahnavi Gollamudi, Swapan Kumar Dasgupta, and Perumal Thiagarajan, "Erythrophagocytosis in Autoimmune Immunoglobulin A-Mediated Hemolysis" (2023). Faculty and Staff Publications. 1069.
https://digitalcommons.library.tmc.edu/baylor_docs/1069
Included in
Biological Phenomena, Cell Phenomena, and Immunity Commons, Cardiology Commons, Cardiovascular Diseases Commons