Publication Date
6-21-2024
Journal
STAR Protocols
DOI
10.1016/j.xpro.2024.103053
PMID
38704833
PMCID
PMC11089399
PubMedCentral® Posted Date
5-4-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Animals, Mice, Imaging, Three-Dimensional, Fluorescent Dyes, Optical Imaging, Developmental biology, microscopy, Model Organisms, Neuroscience
Abstract
Tissue clearing is an essential prerequisite for 3D volumetric imaging of larger tissues or organs. Here, we present a detailed protocol for optical, aqueous-based clearing of adult murine tissues using EZ Clear. We describe steps to ensure successful perfusion and fixation of organs from the adult mouse and supply guidelines for optimal lipid removal, refractive index matching, and tissue clearing. Finally, we provide imaging parameters for visualizing both exogenous perfused fluorescent dyes and endogenous fluorescence reporters in the adult mouse. For complete details on the use and execution of this protocol, please refer to Hsu et al.
Graphical Abstract
Included in
Endocrine System Diseases Commons, Endocrinology, Diabetes, and Metabolism Commons, Neurosciences Commons
Comments
Associated Data