Publication Date
1-20-2022
Journal
Cell
DOI
10.1016/j.cell.2021.12.015
PMID
34982960
PMCID
PMC8928745
PubMedCentral® Posted Date
3-17-2022
PubMedCentral® Full Text Version
Author MSS
Published Open-Access
yes
Keywords
Adaptation, Physiological, Amino Acid Motifs, Amino Acid Sequence, Fluorescence, Molecular Docking Simulation, Nuclear Envelope, Nuclear Pore, Nuclear Pore Complex Proteins, Protein Domains, Reproducibility of Results, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Abstract
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport.
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