Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins

Authors

Cristina Jiménez-Ortigosa
Jennifer Jiang
Muyuan Chen
Xuyuan Kuang
Kelley R Healey
Paul Castellano
Nikpreet Boparai
Steven J Ludtke
David S Perlin
Wei Dai

Publication Date

2-6-2021

Journal

Journal of Fungi

DOI

10.3390/jof7020120

PMID

33562124

PMCID

PMC7914498

PubMedCentral® Posted Date

2-6-2021

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Candida glabrata, fungal membrane proteins, cryo-electron tomography (cryoET)

Abstract

Fungal plasma membrane proteins have long been recognized as targets for the development of antifungal agents. Despite recent progress in experimental approaches and computational structural predictions, our knowledge of the structural dynamics and spatial distribution of these membrane proteins in the context of their native lipid environment remains limited. By applying cryo-electron tomography (cryoET) and subtomogram analysis, we aim to characterize the structural characteristics and spatial distribution of membrane proteins present in Candida glabrata plasma membranes. This study has resulted in the identification of the membrane-embedded structure of the fungal H+-ATPase, Pma1. Tomograms of the plasma membrane revealed that Pma1 complexes are heterogeneously distributed as hexamers that cluster into distinct membrane microdomains. This study characterizes fungal membrane proteins in the native cellular landscape and highlights the unique potential of cryoET to advance our understanding of cellular biology and biological systems.

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