Publication Date
2-18-2020
Journal
The Journal of Infectious Diseases
DOI
10.1093/infdis/jiz526
PMID
31613328
PMCID
PMC8483564
PubMedCentral® Posted Date
10-15-2019
PubMedCentral® Full Text Version
Post-Print
Published Open-Access
yes
Keywords
Adolescent, Adult, Antibodies, Blocking, Antibodies, Neutralizing, Antibodies, Viral, Blood Group Antigens, Caliciviridae Infections, Female, Healthy Volunteers, Humans, Male, Middle Aged, Neutralization Tests, Norovirus, Vaccination, Viral Vaccines, Young Adult, human norovirus, vaccine, neutralization, antibody, immunogenicity, histo-blood group antigen, human intestinal enteroids
Abstract
BACKGROUND: The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels.
METHODS: Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after administration of an investigational norovirus vaccine and were compared with those measured in histo-blood group antigen (HBGA)-blocking assays.
RESULTS: Neutralizing antibody seroresponses were observed in 71% of 24 vaccinated adults, and antibody levels were highly correlated (r = 0.82, P < .001) with those measured by HBGA blocking.
CONCLUSIONS: HBGA-blocking antibodies are a surrogate for neutralization in human noroviruses.
Comments
CLINICAL TRIALS REGISTRATION: NCT02475278.
Associated Data