Publication Date
5-14-2024
Journal
Vaccines
DOI
10.3390/vaccines12050535
PMID
38793786
PMCID
PMC11126127
PubMedCentral® Posted Date
5-14-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
MV140, UTI89, mucosal immunity, vaccine, UTI—urinary tract infection, UPEC—uropathogenic E. coli
Abstract
MV140 is an inactivated whole-cell bacterial mucosal vaccine with proven clinical efficacy against recurrent urinary tract infections (UTIs). These infections are primarily caused by uropathogenic E. coli (UPEC) strains, which are unique in their virulence factors and remarkably diverse. MV140 contains a non-UPEC strain, suggesting that it may induce an immune response against different UPEC-induced UTIs in patients. To verify this, we experimentally evaluated the cellular and humoral responses to UTI89, a prototypical UPEC strain, in mice vaccinated with MV140, as well as the degree of protection achieved in a UPEC UTI89 model of acute cystitis. The results show that both cellular (Th1/Th17) and antibody (IgG/IgA) responses to UTI89 were induced in MV140-immunized mice. MV140 vaccination resulted in an early increased clearance of UTI89 viable bacteria in the bladder and urine following transurethral infection. This was accompanied by a highly significant increase in CD4+ T cells in the bladder and an increase in urinary neutrophils. Collectively, our results support that MV140 induces cross-reactive humoral and cellular immune responses and cross-protection against UPEC strains.
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Allergy and Immunology Commons, Immune System Diseases Commons, Medical Immunology Commons
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