Publication Date
4-26-2022
Journal
mBio
DOI
10.1128/mbio.02848-21
PMID
35297675
PMCID
PMC9040745
PubMedCentral® Posted Date
3-17-2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Animals, Antiviral Agents, Biguanides, Blood Group Antigens, Disinfectants, Gastroenteritis, Humans, Norovirus, Swine, HIEs, HuNoV, IntestiCult media, OLG-HR, antiviral, noroviruses, olanexidine, organoids, virucidal activity
Abstract
Human norovirus (HuNoV) is the leading cause of epidemic and sporadic acute gastroenteritis worldwide. HuNoV transmission occurs predominantly by direct person-to-person contact, and its health burden is associated with poor hand hygiene and a lack of effective antiseptics and disinfectants. Specific therapies and methods to prevent and control HuNoV spread previously were difficult to evaluate because of the lack of a cell culture system to propagate infectious virus. This barrier has been overcome with the successful cultivation of HuNoV in nontransformed human intestinal enteroids (HIEs). Here, we report using the HIE cultivation system to evaluate the virucidal efficacy of an olanexidine gluconate-based hand rub (OLG-HR) and 70% ethanol (EtOH