Publication Date
10-23-2024
Journal
Applied and Environmental Microbiology
DOI
10.1128/aem.01171-24
PMID
39315792
PMCID
PMC11497775
PubMedCentral® Posted Date
9-24-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Gene Library, Bacteriophages, Metagenomics, Virome, Wastewater, Seawater, DNA, Viral, High-Throughput Nucleotide Sequencing, Genome, Viral, Fresh Water, Escherichia coli, metagenomic libraries, DNA libraries, virome, bacteriophage, barcoded DNA, pooled DNA libraries, metagenomics
Abstract
Despite many efforts to understand and leverage the functional potential of environmental viromes, most bacteriophage genes are largely uncharacterized. To explore novel biology from uncultivated microbes like phages, metagenomics has emerged as a powerful tool to directly mine new genes without the need to culture the diverse microbiota and the viruses within. When a pure computational approach cannot infer gene function, it may be necessary to create a DNA library from environmental genomic DNA, followed by the screening of that library for a particular function. However, these screens are often initiated without a metagenomic analysis of the completed DNA library being reported. Here, we describe the construction and characterization of DNA libraries from a single cultured phage (ΦT4), five cultured
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Medical Genetics Commons, Medical Microbiology Commons, Medical Specialties Commons, Virology Commons
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