Publication Date
7-15-2024
Journal
STEM CELLS Translational Medicine
DOI
10.1093/stcltm/szae024
PMID
38584493
PMCID
PMC11227973
PubMedCentral® Posted Date
4-8-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Humans, Mesenchymal Stem Cells, Female, Secretome, Pregnancy, Transcriptome, Gene Expression Profiling, Exosomes, Chorionic Villi, Amniotic Fluid, Cell Line, Tumor, mesenchymal stem cells, gestational tissues, anti-cancer, transcriptomic analysis, secretome
Abstract
The environment created during embryogenesis contributes to reducing aberrations that drive structural malformations and tumorigenesis. In this study, we investigate the anti-cancer effect of mesenchymal stem cells (MSCs) derived from 2 different gestational tissues, the amniotic fluid (AF) and the chorionic villi (CV), with emphasis on their secretome. Transcriptomic analysis was performed on patient-derived AF- and CV-MSCs collected during prenatal diagnosis and identified both mRNAs and lncRNAs, involved in tissue homeostasis and inhibiting biological processes associated with the etiology of aggressive cancers while regulating immune pathways shown to be important in chronic disorders. Secretome enrichment analysis also identified soluble moieties involved in target cell regulation, tissue homeostasis, and cancer cell inhibition through the highlighted Wnt, TNF, and TGF-β signaling pathways. Transcriptomic data were experimentally confirmed through in vitro assays, by evaluating the anti-cancer effect of the media conditioned by AF- and CV-MSCs and the exosomes derived from them on ovarian cancer cells, revealing inhibitory effects in 2D (by reducing cell viability and inducing apoptosis) and in 3D conditions (by negatively interfering with spheroid formation). These data provide molecular insights into the potential role of gestational tissues-derived MSCs as source of anti-cancer factors, paving the way for the development of therapeutics to create a pro-regenerative environment for tissue restoration following injury, disease, or against degenerative disorders.
Included in
Biological Phenomena, Cell Phenomena, and Immunity Commons, Life Sciences Commons, Maternal and Child Health Commons, Medical Cell Biology Commons, Medical Microbiology Commons, Medical Molecular Biology Commons, Oncology Commons, Women's Health Commons
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