Language
English
Publication Date
1-1-2025
Journal
Synthetic Biology
DOI
10.1093/synbio/ysaf015
PMID
40927394
PMCID
PMC12415853
PubMedCentral® Posted Date
8-14-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Modular cloning systems streamline laboratory workflows by consolidating genetic 'parts' into reusable and modular collections, enabling researchers to fast-track strain construction. The GoldenBraid 2.0 modular cloning system utilizes the cutting property of type IIS restriction enzymes to create defined genetic 'grammars', which facilitate the reuse of standardized genetic parts and assembly of genetic parts in the right order. Here, we present a GoldenBraid 2.0 toolkit of genetic parts designed to accelerate cloning in the model bacterium
Published Open-Access
no
Recommended Citation
Cooke, Matthew B; Welch, Kobie T; Ramirez, Laura D; et al., "GoldenBraid2.0 E. coli: A Comprehensive and Characterized Toolkit for Enterics" (2025). Faculty and Staff Publications. 2186.
https://digitalcommons.library.tmc.edu/baylor_docs/2186
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