Publication Date
12-19-2023
Journal
Journal of Bacteriology
DOI
10.1128/jb.00272-23
PMID
38018999
PMCID
PMC10742612
PubMedCentral® Posted Date
11-29-2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Escherichia coli, Escherichia coli Proteins, DNA Repair, Recombination, Genetic, DNA Helicases, endogenous DNA damage, homology-directed DNA repair, chromosome segregation, Escherichia coli, genome integrity, DNA replication, SSB single-strand binding protein, RecG, mutations, evolution
Abstract
In this issue of the Journal of Bacteriology, N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023, https://doi.org/10.1128/jb.00184-23) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins’ identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) “strand-exchange” intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing “death-by-recombination.” DNA damage is managed to regulate crucial but potentially lethal HDR.
Included in
Biological Phenomena, Cell Phenomena, and Immunity Commons, Biomedical Informatics Commons, Genetics and Genomics Commons, Medical Genetics Commons, Medical Molecular Biology Commons, Medical Specialties Commons