Publication Date

10-5-2023

Journal

Genome Biology

DOI

10.1186/s13059-023-03061-1

PMID

37798733

PMCID

PMC10552390

PubMedCentral® Posted Date

10-5-2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Benchmarking, Genomics, Computational Biology, Genome, High-Throughput Nucleotide Sequencing, Genetic variation, SNPs, Indels, Structural variant, Benchmark datasets, Medical genes, Sequencing technology

Abstract

Genomic benchmark datasets are essential to driving the field of genomics and bioinformatics. They provide a snapshot of the performances of sequencing technologies and analytical methods and highlight future challenges. However, they depend on sequencing technology, reference genome, and available benchmarking methods. Thus, creating a genomic benchmark dataset is laborious and highly challenging, often involving multiple sequencing technologies, different variant calling tools, and laborious manual curation. In this review, we discuss the available benchmark datasets and their utility. Additionally, we focus on the most recent benchmark of genes with medical relevance and challenging genomic complexity.

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