De Novo Genome Assembly for the Coppery Titi Monkey (Plecturocebus cupreus): An Emerging Nonhuman Primate Model for Behavioral Research

Authors

Susanne P Pfeifer
Alexander Baxter
Logan E Savidge
Fritz J Sedlazeck
Karen L Bales

Publication Date

5-2-2024

Journal

Genome Biology and Evolution

DOI

10.1093/gbe/evae108

PMID

38758096

PMCID

PMC11140417

PubMedCentral® Posted Date

5-17-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Animals, Pitheciidae, Genome, Genomics, Models, Animal, coppery titi monkey, Plecturocebus cupreus, Pitheciidae, platyrrhine, primate, hybrid assembly

Abstract

The coppery titi monkey (Plecturocebus cupreus) is an emerging nonhuman primate model system for behavioral and neurobiological research. At the same time, the almost entire absence of genomic resources for the species has hampered insights into the genetic underpinnings of the phenotypic traits of interest. To facilitate future genotype-to-phenotype studies, we here present a high-quality, fully annotated de novo genome assembly for the species with chromosome-length scaffolds spanning the autosomes and chromosome X (scaffold N50 = 130.8 Mb), constructed using data obtained from several orthologous short- and long-read sequencing and scaffolding techniques. With a base-level accuracy of ∼99.99% in chromosome-length scaffolds as well as benchmarking universal single-copy ortholog and k-mer completeness scores of >99.0% and 95.1% at the genome level, this assembly represents one of the most complete Pitheciidae genomes to date, making it an invaluable resource for comparative evolutionary genomics research to improve our understanding of lineage-specific changes underlying adaptive traits as well as deleterious mutations associated with disease.

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