Publication Date

10-14-2024

Journal

Genome Biology

DOI

10.1186/s13059-024-03414-4

PMID

39402664

PMCID

PMC11472564

PubMedCentral® Posted Date

10-14-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Genomics, Metagenomics, Humans, Software

Abstract

The exponential increase in sequencing data calls for conceptual and computational advances to extract useful biological insights. One such advance, minimizers, allows for reducing the quantity of data handled while maintaining some of its key properties. We provide a basic introduction to minimizers, cover recent methodological developments, and review the diverse applications of minimizers to analyze genomic data, including de novo genome assembly, metagenomics, read alignment, read correction, and pangenomes. We also touch on alternative data sketching techniques including universal hitting sets, syncmers, or strobemers. Minimizers and their alternatives have rapidly become indispensable tools for handling vast amounts of data.

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