Protocol for the Derivation of Primary Cancer Stem Cell Lines From Human Ependymal Tumors

Authors

Cory M Richman
Peter B Dirks
Michael D Taylor
Kulandaimanuvel Antony Michealraj

Publication Date

9-20-2024

Journal

STAR Protocols

DOI

10.1016/j.xpro.2024.103260

PMID

39153201

PMCID

PMC11378887

PubMedCentral® Posted Date

8-15-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Humans, Neoplastic Stem Cells, Cell Line, Tumor, Ependymoma, Cell Culture Techniques, Brain Neoplasms, Cell Biology, Cancer, Stem Cells, Cell Differentiation

Abstract

Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines. For complete details on the use and execution of this protocol, please refer to Michealraj et al.