Publication Date
1-1-2024
Journal
Frontiers in Cellular Neuroscience
DOI
10.3389/fncel.2024.1404929
PMID
38903773
PMCID
PMC11188360
PubMedCentral® Posted Date
6-5-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
TRPV4, rod bipolar cell, photoreceptor, light response, ERG, patch-clamp, immunocytochemistry, confocal microscopy
Abstract
INTRODUCTION: Mechanical sensitive channels expressed in mammalian retinas are effectors of elevated pressure stresses, but it is unclear how their activation affects visual function in pressure-related retinal disorders.
METHODS: This study investigated the role of the transient potential channel vanilloid TRPV4 in photoreceptors and rod bipolar cells (RBCs) with immunohistochemistry, confocal microscopy, electroretinography (ERG), and patch-clamp techniques.
RESULTS: TRPV4 immunoreactivity (IR) was found in the outer segments of photoreceptors, dendrites and somas of PKCα-positive RBCs and other BCs, plexiform layers, and retinal ganglion cells (RGCs) in wild-type mice. TRPV4-IR was largely diminished in the retinas of homozygous TRPV4 transgenic mice. Genetically suppressing TRPV4 expression moderately but significantly enhanced the amplitude of ERG a- and b-waves evoked by scotopic and mesopic lights (0.55 to 200 Rh*rod
CONCLUSIONS: The data indicates that TRPV4 involves ocular development and is expressed and active in outer retinal neurons, and interventions of TRPV4 can variably affect visual signals in rods, cones, RBCs, and cone ON BCs.