Publication Date
5-9-2023
Journal
Stem Cell Reports
DOI
10.1016/j.stemcr.2023.04.004
PMID
37163980
PMCID
PMC10202694
PubMedCentral® Posted Date
5-9-2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Humans, Mice, Animals, Single-Cell Gene Expression Analysis, Cell Differentiation, Retina, Retinal Cone Photoreceptor Cells, Retinal Degeneration, Organoids, photoreceptor cell, pluripotent stem cell, hereditary retinal diseases, cell invasion, cell motility, transcriptome, neural progenitor
Abstract
Human retinal organoid transplantation could potentially be a treatment for degenerative retinal diseases. How the recipient retina regulates the survival, maturation, and proliferation of transplanted organoid cells is unknown. We transplanted human retinal organoid-derived cells into photoreceptor-deficient mice and conducted histology and single-cell RNA sequencing alongside time-matched cultured retinal organoids. Unexpectedly, we observed human cells that migrated into all recipient retinal layers and traveled long distances. Using an unbiased approach, we identified these cells as astrocytes and brain/spinal cord-like neural precursors that were absent or rare in stage-matched cultured organoids. In contrast, retinal progenitor-derived rods and cones remained in the subretinal space, maturing more rapidly than those in the cultured controls. These data suggest that recipient microenvironment promotes the maturation of transplanted photoreceptors while inducing or facilitating the survival of migratory cell populations that are not normally derived from retinal progenitors. These findings have important implications for potential cell-based treatments of retinal diseases.