Publication Date
5-7-2020
Journal
Nucleic Acids Research
DOI
10.1093/nar/gkaa185
PMID
32232356
PMCID
PMC7192589
PubMedCentral® Posted Date
5-30-2020
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Chemotaxis, Cloning, Molecular, Cyclic AMP, Dictyostelium, Luminescent Proteins, Synthetic Biology
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
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