Language
English
Publication Date
5-7-2020
Journal
Nucleic Acids Research
DOI
10.1093/nar/gkaa185
PMID
32232356
PMCID
PMC7192589
PubMedCentral® Posted Date
5-30-2020
PubMedCentral® Full Text Version
Post-print
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
Keywords
Chemotaxis, Cloning, Molecular, Cyclic AMP, Dictyostelium, Luminescent Proteins, Synthetic Biology
Published Open-Access
yes
Recommended Citation
Kundert, Peter; Sarrion-Perdigones, Alejandro; Gonzalez, Yezabel; et al., "A GoldenBraid Cloning System for Synthetic Biology in Social Amoebae" (2020). Faculty, Staff and Students Publications. 328.
https://digitalcommons.library.tmc.edu/baylor_docs/328