Publication Date
9-17-2021
Journal
STAR Protocols
DOI
10.1016/j.xpro.2021.100783
PMID
34585147
PMCID
PMC8458262
PubMedCentral® Posted Date
9-14-2021
Published Open-Access
no
Keywords
Animals, Animals, Genetically Modified, Drosophila melanogaster, Drug Resistance, Female, Genetic Engineering, Genetic Markers, Male
Abstract
We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate the need to visually screen progeny. Before using these markers in new genetic backgrounds, effective selection/counterselection concentrations should be established for each marker/drug combination. This protocol describes how to set up, perform, and analyze a drug titration curve to determine the effective selection/counterselection drug concentrations for their corresponding markers. For complete details on the use and execution of this protocol, please refer to Matinyan et al., 2021.
Graphical Abstract
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biology Commons, Genomics Commons, Medical Genetics Commons, Medical Specialties Commons, Molecular Genetics Commons
Comments
Associated Data