Language

English

Publication Date

8-15-2023

Journal

Proceedings of the National Academy of Sciences of the United States of America

DOI

10.1073/pnas.2302799120

PMID

37549264

PMCID

PMC10438392

PubMedCentral® Posted Date

8-7-2023

PubMedCentral® Full Text Version

Post-print

Abstract

The nucleobase/ascorbic acid transporter (NAT) family comprises proteins in all kingdoms of life. Their substrates, nucleobases and their analogs, have special attention in therapeutic applications, such as the treatment of solid tumors, lymphoproliferative diseases, viral infections, and inflammatory diseases. Here, we present the crystal structure of a bacterial NAT member, PurT of Colwellia psychrerythraea (PurTCp) at 2.80 Å resolution that, together with functional data, provide insight into the transport mechanism of NAT family members.

Keywords

Animals, Biological Transport, Purines, Mutation, Binding Sites, Ascorbic Acid, Mammals, PurTCp, NAT gene family, H+-coupled transport, nucleobases, X-ray crystallography

Published Open-Access

yes

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