Language
English
Publication Date
1-6-2025
Journal
The Journal of General Physiology
DOI
10.1085/jgp.202413583
PMID
39661086
PMCID
PMC11633665
PubMedCentral® Posted Date
12-11-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Dilated cardiomyopathy (DCM) is a highly prevalent and genetically heterogeneous condition that results in decreased contractility and impaired cardiac function. The FK506-binding protein FKBP12 has been implicated in regulating the ryanodine receptor in skeletal muscle, but its role in cardiac muscle remains unclear. To define the effect of FKBP12 in cardiac function, we generated conditional mouse models of FKBP12 deficiency. We used Cre recombinase driven by either the α-myosin heavy chain, (αMHC) or muscle creatine kinase (MCK) promoter, which are expressed at embryonic day 9 (E9) and E13, respectively. Both conditional models showed an almost total loss of FKBP12 in adult hearts compared with control animals. However, only the early embryonic deletion of FKBP12 (αMHC-Cre) resulted in an early-onset and progressive DCM, increased cardiac oxidative stress, altered expression of proteins associated with cardiac remodeling and disease, and sarcoplasmic reticulum Ca2+ leak. Our findings indicate that FKBP12 deficiency during early development results in cardiac remodeling and altered expression of DCM-associated proteins that lead to progressive DCM in adult hearts, thus suggesting a major role for FKBP12 in embryonic cardiac muscle.
Keywords
Animals, Cardiomyopathy, Dilated, Mice, Tacrolimus Binding Protein 1A, Oxidative Stress, Myocardium, Sarcoplasmic Reticulum, Calcium
Published Open-Access
yes
Recommended Citation
Hanna, Amy D; Chang, Ting; Ho, Kevin S; et al., "Mechanisms Underlying Dilated Cardiomyopathy Associated With FKBP12 Deficiency" (2025). Faculty and Staff Publications. 3978.
https://digitalcommons.library.tmc.edu/baylor_docs/3978