Language
English
Publication Date
2-1-2025
Journal
Experimental & Molecular Medicine
DOI
10.1038/s12276-024-01384-y
PMID
39779976
PMCID
PMC11799519
PubMedCentral® Posted Date
1-8-2025
PubMedCentral® Full Text Version
Post-print
Abstract
The spatial organization of cells within a tissue is dictated throughout dynamic developmental processes. We sought to understand whether cells geometrically coordinate with one another throughout development to achieve their organization. The pancreas is a complex cellular organ with a particular spatial organization. Signals from the mesenchyme, neurons, and endothelial cells instruct epithelial cell differentiation during pancreatic development. To understand the cellular diversity and spatial organization of the developing pancreatic niche, we mapped the spatial relationships between single cells over time. We found that four transcriptionally unique subtypes of mesenchyme in the developing pancreas spatially coordinate throughout development, with each subtype at fixed locations in space and time in relation to other cells, including beta cells, vasculature, and epithelial cells. Our work provides insight into the mechanisms of pancreatic development by showing that cells are organized in a space and time manner.
Keywords
Organogenesis, Pancreas, Animals, Mice, Mesoderm, Gene Expression Regulation, Developmental, Cell Differentiation, Insulin-Secreting Cells, Differentiation, Transgenic organisms
Published Open-Access
yes
Recommended Citation
Scavuzzo, Marissa A; Szlachcic, Wojciech J; Hill, Matthew C; et al., "Pancreatic Organogenesis Mapped Through Space and Time" (2025). Faculty and Staff Publications. 3998.
https://digitalcommons.library.tmc.edu/baylor_docs/3998