Measuring the Acidification of the Phagosomal Lumen in Live C. elegans Embryos

Authors

Omar Peña-Ramos
Zheng Zhou

Publication Date

6-3-2023

Journal

STAR Protocols

DOI

10.1016/j.xpro.2023.102332

PMID

37270784

PMCID

PMC10276153

PubMedCentral® Posted Date

6-3-2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Cell Biology, Developmental Biology, Microscopy, Model Organisms

Abstract

In metazoans, the acidification of the phagosomal lumen is essential for the efficient degradation of cargoes. Here, we present a protocol for measuring the rate of acidification inside phagosomal lumen containing apoptotic cells in living C. elegans embryos. We describe steps for generating a worm population, selecting embryos, and mounting embryos on agar pads. We then detail live imaging of embryos and data analysis. This protocol is applicable to any organism in which real-time fluorescence imaging can be performed. For complete details on the use and execution of this protocol, please refer to Pena-Ramos et al. (2022).

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