Language

English

Publication Date

8-1-2024

Journal

Journal of Diabetes and its Complications

DOI

10.1016/j.jdiacomp.2024.108798

PMID

38991492

PMCID

PMC11288176

PubMedCentral® Posted Date

8-1-2025

PubMedCentral® Full Text Version

Author MSS

Abstract

Aims: Type 1 diabetes has been associated with mitochondrial dysfunction. However, the mechanism of this dysfunction in adults remains unclear.

Methods: A secondary analysis was conducted using data from several clinical trials measuring in-vivo and ex-vivo mitochondrial function in adults with type 1 diabetes (n = 34, age 38.8 ± 14.6 years) and similarly aged controls (n = 59, age 44.6 ± 13.9 years). In-vivo mitochondrial function was assessed before, during, and after isometric exercise with 31phosphorous magnetic resonance spectroscopy. High resolution respirometry of vastus lateralis muscle tissue was used to assess ex-vivo measures.

Results: In-vivo data showed higher rates of anaerobic glycolysis (p = 0.013), and a lower maximal mitochondrial oxidative capacity (p = 0.012) and mitochondrial efficiency (p = 0.024) in adults with type 1 diabetes. After adjustment for age and percent body fat maximal mitochondrial capacity (p = 0.014) continued to be lower and anaerobic glycolysis higher (p = 0.040) in adults with type 1 diabetes. Ex-vivo data did not demonstrate significant differences between the two groups.

Conclusions: The in-vivo analysis demonstrates that adults with type 1 diabetes have mitochondrial dysfunction. This builds on previous research showing in-vivo mitochondrial dysfunction in youths with type 1 diabetes and suggests that defects in substrate or oxygen delivery may play a role in in-vivo dysfunction.

Keywords

Humans, Diabetes Mellitus, Type 1, Adult, Male, Female, Middle Aged, Mitochondria, Muscle, Muscle, Skeletal, Glycolysis, Mitochondrial Diseases, Case-Control Studies, Magnetic Resonance Spectroscopy, Young Adult, Exercise, Mitochondrial function, Muscle, Insulin resistance, High-resolution respirometry, 31phosphorous magnetic resonance spectroscopy

Published Open-Access

yes

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