Language
English
Publication Date
6-14-2023
Journal
Cell Genomics
DOI
10.1016/j.xgen.2023.100298
PMID
37388908
PMCID
PMC10300490
PubMedCentral® Posted Date
4-11-2023
PubMedCentral® Full Text Version
Post-print
Abstract
Cell classes in the human retina are highly heterogeneous with their abundance varying by several orders of magnitude. Here, we generated and integrated a multi-omics single-cell atlas of the adult human retina, including more than 250,000 nuclei for single-nuclei RNA-seq and 137,000 nuclei for single-nuclei ATAC-seq. Cross-species comparison of the retina atlas among human, monkey, mice, and chicken revealed relatively conserved and non-conserved types. Interestingly, the overall cell heterogeneity in primate retina decreases compared with that of rodent and chicken retina. Through integrative analysis, we identified 35,000 distal cis-element-gene pairs, constructed transcription factor (TF)-target regulons for more than 200 TFs, and partitioned the TFs into distinct co-active modules. We also revealed the heterogeneity of the cis-element-gene relationships in different cell types, even from the same class. Taken together, we present a comprehensive single-cell multi-omics atlas of the human retina as a resource that enables systematic molecular characterization at individual cell-type resolution.
Keywords
human retina, single-cell multi-omics, cross-species analysis, gene regulation
Published Open-Access
yes
Recommended Citation
Liang, Qingnan; Cheng, Xuesen; Wang, Jun; et al., "A Multi-Omics Atlas of the Human Retina at Single-Cell Resolution" (2023). Faculty and Staff Publications. 4356.
https://digitalcommons.library.tmc.edu/baylor_docs/4356
Graphical Abstract