Language
English
Publication Date
8-9-2024
Journal
Nature Communications
DOI
10.1038/s41467-024-50853-5
PMID
39117640
PMCID
PMC11310509
PubMedCentral® Posted Date
8-9-2024
PubMedCentral® Full Text Version
Post-print
Abstract
The development of the retina is under tight temporal and spatial control. To gain insights into the molecular basis of this process, we generate a single-nuclei dual-omic atlas of the human developing retina with approximately 220,000 nuclei from 14 human embryos and fetuses aged between 8 and 23-weeks post-conception with matched macular and peripheral tissues. This atlas captures all major cell classes in the retina, along with a large proportion of progenitors and cell-type-specific precursors. Cell trajectory analysis reveals a transition from continuous progression in early progenitors to a hierarchical development during the later stages of cell type specification. Both known and unrecorded candidate transcription factors, along with gene regulatory networks that drive the transitions of various cell fates, are identified. Comparisons between the macular and peripheral retinae indicate a largely consistent yet distinct developmental pattern. This atlas offers unparalleled resolution into the transcriptional and chromatin accessibility landscapes during development, providing an invaluable resource for deeper insights into retinal development and associated diseases.
Keywords
Humans, Retina, Gene Expression Regulation, Developmental, Single-Cell Analysis, Gene Regulatory Networks, Transcription Factors, Cell Differentiation, Fetus, Cell Nucleus, Atlases as Topic, Developmental neurogenesis, Differentiation
Published Open-Access
yes
Recommended Citation
Zuo, Zhen; Cheng, Xuesen; Ferdous, Salma; et al., "Single Cell Dual-Omic Atlas of the Human Developing Retina" (2024). Faculty and Staff Publications. 4361.
https://digitalcommons.library.tmc.edu/baylor_docs/4361