Cell Type Composition and Circuit Organization of Clonally Related Excitatory Neurons in the Juvenile Mouse Neocortex

Authors

Cathryn R Cadwell
Federico Scala
Paul G Fahey
Dmitry Kobak
Shalaka Mulherkar
Fabian H Sinz
Stelios Papadopoulos
Zheng H Tan
Per Johnsson
Leonard Hartmanis
Shuang Li
Ronald J Cotton
Kimberley F Tolias
Rickard Sandberg
Philipp Berens
Xiaolong Jiang
Andreas Savas Tolias

Publication Date

3-5-2020

Journal

eLife

DOI

10.7554/eLife.52951

PMID

32134385

PMCID

PMC7162653

PubMedCentral® Posted Date

3-5-2020

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Animals, Cells, Cultured, Mice, Neocortex, Neurons, Synapses

Abstract

Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize the cell types and circuit diagram of clonally related excitatory neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from Nestin-positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are derived from two radial glia on average, span cortical layers 2–6, and are composed of a random sampling of transcriptomic cell types. We find an interaction between shared lineage and connection type: related neurons are more likely to be connected vertically across cortical layers, but not laterally within the same layer. These findings challenge the view that related neurons show uniformly increased connectivity and suggest that integration of vertical intra-clonal input with lateral inter-clonal input may represent a developmentally programmed connectivity motif supporting the emergence of functional circuits.

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