Language

English

Publication Date

4-1-2025

Journal

Mucosal Immunology

DOI

10.1016/j.mucimm.2025.01.004

Abstract

First- and secondhand smokers are at an increased risk for influenza virus (IFV)-related respiratory failure and death. Despite approved influenza antiviral treatments, there is an unmet need for treatments that can improve outcomes in populations at risk for respiratory failure, including tobacco users with Chronic Obstructive Pulmonary Disease (COPD). Here we show that the sialidase fusion protein, DAS181, reduced viral burden, mitigated inflammation, and attenuated lung function loss, consistent with broad-spectrum anti-influenza responses in a mouse model of COPD and IFV-A infection. Treatment with DAS181 reprogramed the sialic acid-binding immunoglobulin-like lectins (Siglecs) in alveolar macrophages, increased expression of phagocytic marker CD169, and downregulated inhibitory Siglec-F and Siglec-H molecules. Upon reinfection, mice treated with DAS181 showed activated and protective memory response in the lungs. Collectively, we show that this sialidase fusion protein promotes a beneficial immunomodulatory reaction in the lungs, supporting a new IFV-A therapeutic option for at-risk smokers.

Keywords

Animals, Pulmonary Disease, Chronic Obstructive, Neuraminidase, Mice, Disease Models, Animal, Orthomyxoviridae Infections, Humans, Recombinant Fusion Proteins, Sialic Acid Binding Immunoglobulin-like Lectins, Macrophages, Alveolar, Lung, Mice, Inbred C57BL, Cigarette Smoking, Influenza A virus, Smoke, Influenza, Human, Alveolar macrophage, Inflammation, Influenza infection, Sialidase fusion protein, Siglec

Published Open-Access

yes

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