Language
English
Publication Date
10-30-2025
Journal
American Journal of Physiology: Gastrointestinal and Liver Physiology
DOI
10.1152/ajpgi.00186.2025
PMID
41165566
Abstract
Radiation exposure impairs rapidly renewing tissues like the intestinal epithelium, yet translational insights from murine models have been limited by species-specific responses. Here, we use human intestinal organoids (HIOs) derived from jejunal epithelium to evaluate human epithelial responses to low dose proton and photon (gamma) radiation. Gamma irradiation induces a unique developmental and metabolic shift in crypt-like organoids, including enrichment of amino acid metabolism pathways and activation of fetal-associated transcription factors and morphology. Integrated multi-omic profiling reveals serotonin biosynthesis as a central regenerative node. Human intestinal organoids can complement animal models and are emerging as a powerful tool in modeling human radiation responses and identifying candidate biomarkers for intestinal injury.
Keywords
: intestine, organoid, radiation, stem cell reversion
Published Open-Access
yes
Recommended Citation
Poplaski, Victoria; Patel, Tajhal D; Nguyen-Phuc, Hoa; et al., "Distinct Molecular Responses of Human Intestinal Organoids to Proton and Photon Radiation" (2025). Faculty, Staff and Students Publications. 4702.
https://digitalcommons.library.tmc.edu/baylor_docs/4702