Language

English

Publication Date

7-11-2025

Journal

Infectious Diseases of Poverty

DOI

10.1186/s40249-025-01334-4

PMID

40646638

PMCID

PMC12247418

PubMedCentral® Posted Date

7-11-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Lowndes County is a predominantly Black rural county in Alabama, in the United States, which has a historical and current legacy of racial discrimination, creating inequitable infrastructure access and adverse health impacts. Over 80% rely on on-site sanitation infrastructure and most are failing. A community assessment of exposure to untreated sewage was conducted using samples from residential drinking water, surface swabs, and soil combined with environmental water and soil samples using culture-based and quantitative polymerase chain reaction (qPCR) methods. Testing varied slightly across samples, due to difficulty of access or availability. Of 43 households, 68% and 55% of houses had detectable presence of human fecal matter indicator in their soils and on their doorsteps, respectively, and 0% had detectable amounts of culturable Escherichia coli in their drinking water. Of 40 houses sampled, 88% tested positive for E. coli in soil samples. Of 39 residences, 31% had positive presence of environmental and zoonotic parasites in soil, but none for Necator americanus, Cryptosporidium species, or Giardia intestinalis. Of the 18 sampled environmental surface waters, 100% tested positive for culturable E. coli, 50% had detectable human fecal matter indicator present, and 27% tested positive for anthropogenic parasites. This work sheds light that there is presence of culturable E. coli, human fecal matter, and anthropogenic parasites in residential soil samples of all sanitation types (municipal, septic tank, and straight piping) and in environmental surface waters throughout the sampled areas. Our findings support the narrative that sanitation infrastructure of all types in Lowndes County, Alabama are compromised and highlights residential and environmental exposure to raw wastewater.

Keywords

Alabama, Humans, Sanitation, Sewage, Feces, Escherichia coli, Soil, Parasites, Drinking Water, Animals, Soil Microbiology

Published Open-Access

yes

40249_2025_1334_Figa_HTML.jpg (1012 kB)
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