Language

English

Publication Date

7-1-2025

Journal

Advanced Science

DOI

10.1002/advs.202504364

PMID

40323169

PMCID

PMC12279244

PubMedCentral® Posted Date

5-5-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Synthetic biology has enabled the development of biosensors to detect intestinal inflammation, yet few target the clinically validated biomarker of intestinal inflammation calprotectin with both diagnostic and therapeutic capabilities. Here, an optimized calprotectin biosensor is presented that leverages a zinc uptake regulator (Zur) controlled promoter coupled with a memory circuit to detect and record intestinal inflammation in vivo. The level of biosensor activation strongly correlates with calprotectin levels in the colon of two independent mouse models of colitis. Coupling of the biosensor with the production of the anti-inflammatory cytokine IL10 allowed for the resolution of chemically induced colitis, demonstrating the ability of the biosensor to sense and respond to disease. This work highlights the utility of developing synthetic organisms for the diagnosis and treatment of intestinal disease using clinically validated biomarkers.

Keywords

Biosensing Techniques, Animals, Mice, Colitis, Leukocyte L1 Antigen Complex, Disease Models, Animal, Inflammation, Biomarkers, Mice, Inbred C57BL, biosensors, calprotectin, IL10, intestinal inflammation, Zur

Published Open-Access

yes

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