Language
English
Publication Date
5-27-2024
Journal
Endocrinology
DOI
10.1210/endocr/bqae070
PMID
38916490
PMCID
PMC11210311
PubMedCentral® Posted Date
6-25-2024
PubMedCentral® Full Text Version
Post-print
Abstract
The epithelial cell lining of the oviduct plays an important role in oocyte pickup, sperm migration, preimplantation embryo development, and embryo transport. The oviduct epithelial cell layer comprises ciliated and nonciliated secretory cells. The ciliary function has been shown to support gamete and embryo movement in the oviduct, yet secretory cell function has not been well characterized. Therefore, our goal was to generate a secretory cell-specific Cre recombinase mouse model to study the role of the oviductal secretory cells. A knock-in mouse model, Ovgp1Cre:eGFP, was created by expressing Cre from the endogenous Ovgp1 (oviductal glycoprotein 1) locus, with enhanced green fluorescent protein (eGFP) as a reporter. EGFP signals were strongly detected in the secretory epithelial cells of the oviducts at estrus in adult Ovgp1Cre:eGFP mice. Signals were also detected in the ovarian stroma, uterine stroma, vaginal epithelial cells, epididymal epithelial cells, and elongated spermatids. To validate recombinase activity, progesterone receptor (PGR) expression was ablated using the Ovgp1Cre:eGFP; Pgrf/f mouse model. Surprisingly, the deletion was restricted to the epithelial cells of the uterotubal junction (UTJ) region of Ovgp1Cre:eGFP; Pgrf/f oviducts. Deletion of Pgr in the epithelial cells of the UTJ region had no effect on female fecundity. In summary, we found that eGFP signals were likely specific to secretory epithelial cells in all regions of the oviduct. However, due to a potential target-specific Cre activity, validation of appropriate recombination and expression of the gene(s) of interest is absolutely required to confirm efficient deletion when generating conditional knockout mice using the Ovgp1Cre:eGFP line.
Keywords
Animals, Female, Mice, Epithelial Cells, Integrases, Glycoproteins, Male, Oviducts, Mice, Transgenic, Green Fluorescent Proteins, Fallopian Tubes, Receptors, Progesterone, Models, Animal, Cre-recombinase, oviduct, ovgp1, progesterone receptor, secretory epithelial cell, uterotubal junction
Published Open-Access
yes
Recommended Citation
McGlade, Emily A; Mao, Jiude; Stephens, Kalli K; et al., "Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct" (2024). Faculty and Staff Publications. 4885.
https://digitalcommons.library.tmc.edu/baylor_docs/4885
Included in
Endocrinology, Diabetes, and Metabolism Commons, Health Services Research Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons, Microbiology Commons