Publication Date

11-1-2025

Journal

Genetics in Medicine

DOI

10.1016/j.gim.2025.101555

PMID

40819229

Abstract

Purpose: XPO1 functions in key cellular processes, including nucleo-cytoplasmic export and mitosis. The gene is deleted in a subset of patients with the 2p15p16.1 microdeletion syndrome; however, no monogenic XPO1-related disorder has been described to date.

Methods: We collected clinical data of individuals with de novo XPO1 variants through online matchmaking. We used Drosophila to study XPO1 function in development and habituation learning.

Results: A total of 22 individuals met the criteria to be included in the main study cohort. Of these, half have putative loss-of-function variants, and half have coding variants (10 missense and 1 in-frame deletion variant). We found an overlapping phenotype, consistent with a monogenic neurodevelopmental disorder. We demonstrate XPO1 functions in development by ubiquitous and neuron-specific knockdown in Drosophila. GABAergic neuron specific knockdown flies demonstrated impaired habituation.

Conclusion: Our results establish XPO1 as a novel dominant monogenic neurodevelopmental disorder gene and demonstrate a central role for XPO1 in development.

Keywords

Exportin 1 Protein, Humans, Receptors, Cytoplasmic and Nuclear, Karyopherins, Animals, Neurodevelopmental Disorders, Female, Male, Child, Drosophila, Phenotype, Drosophila melanogaster, Adult, Adolescent, Child, Preschool, Dominant inheritance. Habituation. Mendelian disorders. Monogenic NDD. XPO1

Published Open-Access

yes

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