Language
English
Publication Date
4-1-2025
Journal
American Journal of Physiology: Heart and Circulatory Physiology
DOI
10.1152/ajpheart.00806.2024
PMID
40062653
Abstract
We investigated ferroptosis, a type of programmed cell death mechanism, in human hearts donated after brain death (DBD) and those donated after circulatory death (DCD), focusing on warm ischemia time (WIT) and cold storage. A total of 24 hearts were procured, with six from the DBD group and 18 from the DCD group. The DCD group was divided into three subgroups, each containing six hearts, based on different WITs of 20, 40, and 60 min. All procured hearts were placed in cold storage for up to 6 h. Left ventricular biopsies were performed at 0, 2, 4, and 6 h. We measured ferroptosis regulators [glutathione peroxidase 4 (GPX4), acyl-CoA synthetase long chain family member 4 (ACSL4), and transferrin receptor], iron content (Fe
Keywords
Humans, Ferroptosis, Warm Ischemia, Organ Preservation, Heart Transplantation, Middle Aged, Male, Adult, Myocardium, Female, Brain Death, Iron, Lipid Peroxidation, Time Factors, Aged, Cold Ischemia, Coenzyme A Ligases, Phospholipid Hydroperoxide Glutathione Peroxidase, cold ischemia injury, ferroptosis, heart transplantation, human donor heart, warm ischemia injury
Published Open-Access
yes
Recommended Citation
Li, Shiyi; Nordick, Katherine V; Elsenousi, Abdussalam E; et al., "Warm-Ischemia and Cold Storage Induced Modulation of Ferroptosis Observed in Human Hearts Donated After Circulatory Death and Brain Death" (2025). Faculty and Staff Publications. 4960.
https://digitalcommons.library.tmc.edu/baylor_docs/4960
Included in
Health Services Research Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons, Medical Specialties Commons, Microbiology Commons