Language
English
Publication Date
12-1-2022
Journal
Virulence
DOI
10.1080/21505594.2022.2123302
PMID
36121102
PMCID
PMC9487758
PubMedCentral® Posted Date
9-19-2022
PubMedCentral® Full Text Version
Post-print
Abstract
To investigate the role of adrenergic signalling (AS) in the host immune response and Porphyromonas gingivalis virulence, we compared norepinephrine (NE) and isoproterenol (ISO) responses in Galleria mellonella. P. gingivalis infection was evaluated by survival; humoral immune responses (i.e. melanization and cecropin and gloverin mRNA expression); cellular immune responses (i.e. haemocyte count, nodulation by histology); and P. gingivalis recovery (CFU/mL). P. gingivalis was cultivated in the presence of ISO (PgISO) or NE and injected into the larvae for survival evaluation. Finally, we co-injected ISO and PgISO to evaluate the concomitant effects on the immune response and bacterial virulence. None of the ligands were toxic to the larvae; ISO increased haemocyte number, even after P. gingivalis infection, by mobilizing sessile haemocytes in a β-adrenergic-specific manner, while NE showed the opposite effect. ISO treatment reduced larval mortality and the number of recovered bacteria, while NE increased mortality and showed no effect on bacterial recovery. ISO and NE had similar effects on melanization and decreased the expression of cecropin. Although co-cultivation with NE and ISO increased the gene expression of bacterial virulence factors in vitro, only the injection of PgISO increased larval death, which was partially reversed by circulating ISO. Therefore, α- and β-adrenergic signalling had opposite effects after P. gingivalis infection. Ultimately, the catecholamine influence on the immune response overcame the effect of more virulent strains. The effect of AS directly on the pathogen found in vitro did not translate to the in vivo setting.
Keywords
Adrenergic Agents, Animals, Cecropins, Immunity, Innate, Isoproterenol, Larva, Moths, Norepinephrine, Porphyromonas gingivalis, RNA, Messenger, Virulence, Virulence Factors, Galleria mellonella, Porphyromonas gingivalis, adrenergic signalling, invertebrates, innate immunity
Published Open-Access
yes
Recommended Citation
Moraes, Renata Mendonça; Garcia, Maíra Terra; Stossi, Fabio; et al., "Effects of α and β-Adrenergic Signaling on Innate Immunity and Porphyromonas gingivalis Virulence in an Invertebrate Model" (2022). Faculty and Staff Publications. 4971.
https://digitalcommons.library.tmc.edu/baylor_docs/4971
Included in
Health Services Research Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons, Medical Specialties Commons, Microbiology Commons