Language

English

Publication Date

3-15-2024

Journal

Development

DOI

10.1242/dev.202354

PMID

38421307

PMCID

PMC11006390

PubMedCentral® Posted Date

3-28-2024

PubMedCentral® Full Text Version

Post-print

Abstract

Interactions between notochord and sclerotome are required for normal embryonic spine patterning, but whether the postnatal derivatives of these tissues also require interactions for postnatal intervertebral disc (IVD) growth and maintenance is less established. We report here the comparative analysis of four conditional knockout mice deficient for TonEBP, a transcription factor known to allow cells to adapt to changes in extracellular osmotic pressure, in specific compartments of the IVD. We show that TonEBP deletion in nucleus pulposus (NP) cells does not affect their survival or aggrecan expression, but promoted cell proliferation in the NP and in adjacent vertebral growth plates (GPs). In cartilage end plates/GPs, TonEBP deletion induced cell death, but also structural alterations in the adjacent NP cells and vertebral bodies. Embryonic or postnatal TonEBP loss generated similar IVD changes. In addition to demonstrating the requirement of TonEBP in the different compartments of the IVD, this comparative analysis uncovers the in vivo interdependency of the different IVD compartments during the growth of the postnatal IVD-vertebral units.

Keywords

Animals, Mice, Gene Expression Regulation, Intervertebral Disc, Mice, Knockout, Osmotic Pressure, Transcription Factors, NFATC Transcription Factors, Nfat5, TonEBP, Intervertebral disc, Mouse

Published Open-Access

yes

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