Language

English

Publication Date

4-9-2025

Journal

The Journal of Neuroscience

DOI

10.1523/JNEUROSCI.2103-24.2025

PMID

39984203

PMCID

PMC11984096

PubMedCentral® Posted Date

2-21-2025

PubMedCentral® Full Text Version

Post-print

Abstract

The mammalian cochlea receives efferent feedback from the brain. Many functions for this feedback have been hypothesized, including on short timescales, such as mediating attentional states, and long timescales, such as buffering acoustic trauma. Testing these hypotheses has been impeded by an inability to make direct measurements of efferent effects in awake animals. Here, we assessed the role of the medial olivocochlear (MOC) efferent nerve fibers on cochlear amplification by measuring organ of Corti vibratory responses to sound in both sexes of awake and anesthetized mice. We studied long-term effects by genetically ablating the efferents and/or afferents. Cochlear amplification increased with deafferentation using VGLUT3−/− mice, but only when the efferents were intact, associated with increased activity within OHCs and supporting cells. Removing both the afferents and the efferents using VGLUT3−/− Alpha9−/− mice did not cause this effect. To test for short-term effects, we recorded sound-evoked vibrations while using pupillometry to measure neuromodulatory brain state. We found no state dependence of cochlear amplification or of the auditory brainstem response. However, state dependence was apparent in the downstream inferior colliculus. Thus, MOC efferents upregulate cochlear amplification chronically with hearing loss, but not acutely with brain state fluctuations. This pathway may partially compensate for hearing loss while mediating associated symptoms, such as tinnitus and hyperacusis.

Keywords

Animals, Mice, Cochlea, Efferent Pathways, Male, Female, Olivary Nucleus, Hearing Loss, Mice, Knockout, Acoustic Stimulation, Mice, Inbred C57BL, Evoked Potentials, Auditory, Brain Stem, Amino Acid Transport Systems, Acidic, brain state, cochlea, feedback, hearing, optical coherence tomography, outer hair cell, pupillometry

Published Open-Access

yes

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.