Language
English
Publication Date
10-14-2024
Journal
Cancer Cell
DOI
10.1016/j.ccell.2024.08.009
PMID
39241781
PMCID
PMC11479845
PubMedCentral® Posted Date
10-14-2025
PubMedCentral® Full Text Version
Author MSS
Abstract
Prior studies have described the complex interplay that exists between glioma cells and neurons, however, the electrophysiological properties endogenous to tumor cells remain obscure. To address this, we employed Patch-sequencing on human glioma specimens and found that one third of patched cells in IDH mutant (IDHmut) tumors demonstrate properties of both neurons and glia by firing single, short action potentials. To define these hybrid cells (HCs) and discern if they are of tumoral origin, we developed a computational tool, Single Cell Rule Association Mining (SCRAM), to annotate each cell individually. SCRAM revealed that HCs represent tumor and non-tumor cells that have select features of GABAergic neurons and oligodendrocyte precursor cells. These studies characterize the combined electrophysiological and molecular properties of human glioma cells and describe a cell type in human glioma with unique electrophysiological and transcriptomic properties that may also exist in the non-tumor brain.
Keywords
Humans, Glioma, Brain Neoplasms, Single-Cell Analysis, Action Potentials, Mutation, Genomics, Transcriptome, Isocitrate Dehydrogenase, GABAergic Neurons, Electrophysiological Phenomena
Published Open-Access
yes
Recommended Citation
Curry, Rachel N; Ma, Qianqian; McDonald, Malcolm F; et al., "Integrated Electrophysiological and Genomic Profiles of Single Cells Reveal Spiking Tumor Cells in Human Glioma" (2024). Faculty and Staff Publications. 5301.
https://digitalcommons.library.tmc.edu/baylor_docs/5301