Language
English
Publication Date
1-6-2023
Journal
Journal of Visualized Experiments
DOI
10.3791/64448
PMID
36688555
PMCID
PMC10208800
PubMedCentral® Posted Date
1-6-2024
PubMedCentral® Full Text Version
Author MSS
Abstract
Endometrial tissue lines the inner cavity of the uterus and is under the cyclical control of estrogen and progesterone. It is a tissue that is composed of luminal and glandular epithelium, a stromal compartment, a vascular network, and a complex immune cell population. Mouse models have been a powerful tool to study the endometrium, revealing critical mechanisms that control implantation, placentation, and cancer. The recent development of 3D endometrial organoid cultures presents a state-of-the-art model to dissect the signaling pathways that underlie endometrial biology. Establishing endometrial organoids from genetically engineered mouse models, analyzing their transcriptomes, and visualizing their morphology at a single-cell resolution are crucial tools for the study of endometrial diseases. This paper outlines methods to establish 3D cultures of endometrial epithelium from mice and describes techniques to quantify gene expression and analyze the histology of the organoids. The goal is to provide a resource that can be used to establish, culture, and study the gene expression and morphological characteristics of endometrial epithelial organoids.
Keywords
Pregnancy, Female, Mice, Animals, Endometrium, Uterus, Epithelium, Estrogens, Organoids, endometrium, organoids, infertility, uterus, regeneration
Published Open-Access
yes
Recommended Citation
Tang, Suni; Parks, Sydney E; Liao, Zian; et al., "Establishing 3D Endometrial Organoids from the Mouse Uterus" (2023). Faculty and Staff Publications. 5662.
https://digitalcommons.library.tmc.edu/baylor_docs/5662
Included in
Allergy and Immunology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Pathology Commons