Language
English
Publication Date
9-15-2023
Journal
STAR Protocols
DOI
10.1016/j.xpro.2023.102540
PMID
37682718
PMCID
PMC10493592
PubMedCentral® Posted Date
9-7-2023
PubMedCentral® Full Text Version
Post-print
Abstract
We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, collecting cell-free supernatant, litmus dye addition, and pH-based calibration curve preparations. This assay has been designed for flexible formatting that can accommodate both high-volume and low-volume sample sets.
Keywords
Spectrophotometry, Spectrophotometry, Ultraviolet, Light, Calibration, Hydrogen-Ion Concentration, High Throughput Screening, Microbiology
Published Open-Access
yes
Recommended Citation
Engevik, Kristen A; Gonzalez, Hyland; Daniels, Clay; et al., "A High-Throughput Protocol for Measuring Solution PH of Bacterial Cultures Using Uv-Vis Absorption Spectrophotometry" (2023). Faculty and Staff Publications. 5683.
https://digitalcommons.library.tmc.edu/baylor_docs/5683
Graphical Abstract
Included in
Allergy and Immunology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Pathology Commons