Language
English
Publication Date
12-16-2024
Journal
The Journal of Infectious Diseases
DOI
10.1093/infdis/jiae282
PMID
38798080
PMCID
PMC11646618
PubMedCentral® Posted Date
5-27-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Background: Recently, face mask sampling (FMS) confirmed detection of Mycobacterium tuberculosis DNA from exhaled breath in adults with tuberculosis. To date, no study has evaluated the use of FMS to detect pulmonary tuberculosis in children. We developed a method for FMS of M. tuberculosis-specific DNA in children and performed a clinical exploration to assess feasibility in children.
Methods: Face masks were spiked, analyzed on GeneXpert-Ultra, quantitative polymerase chain reaction, and targeted next-generation sequencing. Children with pulmonary tuberculosis were asked to wear 3 modified FFP2 masks for 30 minutes as part of an exploratory clinical study.
Results: Experiments with H37Ra M. tuberculosis strain showed a limit of 95% detection of 3.75 colony-forming units (95% confidence interval, 4.85-3.11) on GeneXpert-Ultra. Ten children with pulmonary tuberculosis participated in the clinical study. M. tuberculosis-specific DNA was detected on none of the face masks.
Conclusions: Pediatric FMS has a low limit of detection for M. tuberculosis-specific DNA in vitro. However, M. tuberculosis DNA was not detected in any of 30 masks worn by children with pulmonary tuberculosis. This suggests that FMS in this form may not be more effective for detecting M. tuberculosis in children with tuberculosis than existing methods.
Keywords
Humans, Tuberculosis, Pulmonary, Mycobacterium tuberculosis, Child, Masks, Male, Female, DNA, Bacterial, Child, Preschool, Adolescent, High-Throughput Nucleotide Sequencing
Published Open-Access
yes
Recommended Citation
Meiwes, Lennard; Kontsevaya, Irina; Chesov, Dumitru; et al., "Whispers in the Wind: Face Mask Sampling for Mycobacterium tuberculosis Detection in Children With Pulmonary Tuberculosis" (2024). Faculty, Staff and Students Publications. 5858.
https://digitalcommons.library.tmc.edu/baylor_docs/5858