Language
English
Publication Date
9-1-2023
Journal
Life Science Alliance
DOI
10.26508/lsa.202201683
PMID
37414528
PMCID
PMC10326419
PubMedCentral® Posted Date
7-6-2023
PubMedCentral® Full Text Version
Post-print
Abstract
Members of the BTB-ZF transcription factor family regulate the immune system. Our laboratory identified that family member Zbtb20 contributes to the differentiation, recall responses, and metabolism of CD8 T cells. Here, we report a characterization of the transcriptional and epigenetic signatures controlled by Zbtb20 at single-cell resolution during the effector and memory phases of the CD8 T cell response. Without Zbtb20, transcriptional programs associated with memory CD8 T cell formation were up-regulated throughout the CD8 T response. A signature of open chromatin was associated with genes controlling T cell activation, consistent with the known impact on differentiation. In addition, memory CD8 T cells lacking Zbtb20 were characterized by open chromatin regions with overrepresentation of AP-1 transcription factor motifs and elevated RNA- and protein-level expressions of the corresponding AP-1 components. Finally, we describe motifs and genomic annotations from the DNA targets of Zbtb20 in CD8 T cells identified by cleavage under targets and release under nuclease (CUT&RUN). Together, these data establish the transcriptional and epigenetic networks contributing to the control of CD8 T cell responses by Zbtb20.
Keywords
Transcription Factor AP-1, Gene Expression Regulation, Cell Differentiation, CD8-Positive T-Lymphocytes, Chromatin
Published Open-Access
yes
Recommended Citation
Preiss, Nicholas K; Kamal, Yasmin; Wilkins, Owen M; et al., "Characterizing Control of Memory CD8 T Cell Differentiation by Btb-Zf Transcription Factor Zbtb20" (2023). Faculty, Staff and Students Publications. 6220.
https://digitalcommons.library.tmc.edu/baylor_docs/6220