Language
English
Publication Date
1-1-2023
Journal
Nature Structural & Molecular Biology
DOI
10.1038/s41594-022-00880-x
PMID
36522426
PMCID
PMC10513740
PubMedCentral® Posted Date
9-21-2023
PubMedCentral® Full Text Version
Author MSS
Abstract
The mechanism controlling the dynamic targeting of SWI/SNF has long been postulated to be coordinated by transcription factors (TFs), yet demonstrating a specific TF influence has proven difficult. Here we take a multi-omics approach to interrogate transient SWI/SNF interactors, chromatin targeting and the resulting three-dimensional epigenetic landscape. We utilize the labeling technique TurboID to map the SWI/SNF interactome and identify the activator protein-1 (AP-1) family members as critical interacting partners for SWI/SNF complexes. CUT&RUN profiling demonstrates SWI/SNF targeting enrichment at AP-1 bound loci, as well as SWI/SNF-AP-1 cooperation in chromatin targeting. HiChIP reveals AP-1-SWI/SNF-dependent restructuring of the three-dimensional promoter-enhancer architecture and generation of enhancer hubs. Through interrogation of the SWI/SNF-AP-1 interaction, we demonstrate an SWI/SNF dependency on AP-1-mediated chromatin localization. We propose that pioneer factors, such as AP-1, bind and target SWI/SNF to inactive chromatin, where it restructures the genomic landscape into an active state through epigenetic rewiring spanning multiple dimensions.
Keywords
Transcription Factor AP-1, Chromatin, Chromatin Assembly and Disassembly, Nuclear Proteins, Promoter Regions, Genetic
Published Open-Access
yes
Recommended Citation
Wolf, Bennett K; Zhao, Yanding; McCray, Andrew; et al., "Cooperation of Chromatin Remodeling Swi/Snf Complex and Pioneer Factor Ap-1 Shapes 3D Enhancer Landscapes" (2023). Faculty, Staff and Students Publications. 6224.
https://digitalcommons.library.tmc.edu/baylor_docs/6224