Mesoscopic Calcium Imaging in a Head-Unrestrained Male Non-Human Primate Using a Lensless Microscope
Language
English
Publication Date
2-10-2024
Journal
Nature Communications
DOI
10.1038/s41467-024-45417-6
PMID
38341403
PMCID
PMC10858944
PubMedCentral® Posted Date
2-10-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Mesoscopic calcium imaging enables studies of cell-type specific neural activity over large areas. A growing body of literature suggests that neural activity can be different when animals are free to move compared to when they are restrained. Unfortunately, existing systems for imaging calcium dynamics over large areas in non-human primates (NHPs) are table-top devices that require restraint of the animal’s head. Here, we demonstrate an imaging device capable of imaging mesoscale calcium activity in a head-unrestrained male non-human primate. We successfully miniaturize our system by replacing lenses with an optical mask and computational algorithms. The resulting lensless microscope can fit comfortably on an NHP, allowing its head to move freely while imaging. We are able to measure orientation columns maps over a 20 mm2 field-of-view in a head-unrestrained macaque. Our work establishes mesoscopic imaging using a lensless microscope as a powerful approach for studying neural activity under more naturalistic conditions.
Keywords
Male, Animals, Calcium, Microscopy, Primates, Fluorescence imaging, Optical imaging, Ca2+ imaging, Wide-field fluorescence microscopy, Visual system
Published Open-Access
yes
Recommended Citation
Wu, Jimin; Chen, Yuzhi; Veeraraghavan, Ashok; et al., "Mesoscopic Calcium Imaging in a Head-Unrestrained Male Non-Human Primate Using a Lensless Microscope" (2024). Faculty, Staff and Students Publications. 6288.
https://digitalcommons.library.tmc.edu/baylor_docs/6288