Language

English

Publication Date

9-17-2024

Journal

Molecular Pharmacology

DOI

10.1124/molpharm.124.000889

PMID

39168657

PMCID

PMC11413914

PubMedCentral® Posted Date

10-1-2024

PubMedCentral® Full Text Version

Post-print

Abstract

Autophagy is an essential self-degradative and recycling mechanism that maintains cellular homeostasis. Estrogen receptor-related orphan receptors (ERRs) are fundamental in regulating cardiac metabolism and function. Previously, we showed that ERR agonists improve cardiac function in models of heart failure and induce autophagy. Here, we characterized a mechanism by which ERRs induce the autophagy pathway in cardiomyocytes. Transcription factor EB (TFEB) is a master regulator of the autophagy-lysosome pathway and has been shown to be crucial regulator of genes that control autophagy. We discovered that TFEB is a direct ERR target gene whose expression is induced by ERR agonists. Activation of ERR results in increased TFEB expression in both neonatal rat ventricular myocytes and C2C12 myoblasts. An ERR-dependent increase in TFEB expression results in increased expression of an array of TFEB target genes, which are critical for the stimulation of autophagy. Pharmacologically targeting ERR is a promising potential method for the treatment of many diseases where stimulation of autophagy may be therapeutic, including heart failure.

Keywords

Animals, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Autophagy, Receptors, Estrogen, Rats, Mice, Myocytes, Cardiac, Humans, Cell Line, ERRalpha Estrogen-Related Receptor, Rats, Sprague-Dawley

Published Open-Access

yes

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