Language

English

Publication Date

4-1-2023

Journal

Advanced Science

DOI

10.1002/advs.202206435

PMID

36721029

PMCID

PMC10074073

PubMedCentral® Posted Date

1-31-2023

PubMedCentral® Full Text Version

Post-print

Abstract

The first line of treatment for most solid tumors is surgical resection of the primary tumor with adequate negative margins. Incomplete tumor resections with positive margins account for over 75% of local recurrences and the development of distant metastases. In cases of oral cavity squamous cell carcinoma (OSCC), the rate of successful tumor removal with adequate margins is just 50–75%. Advanced real‐time imaging methods that improve the detection of tumor margins can help improve success rates,overall safety, and reduce the cost. Fluorescence imaging in the second near‐infrared (NIR‐II) window has the potential to revolutionize the field due to its high spatial resolution, low background signal, and deep tissue penetration properties, but NIR‐II dyes with adequate in vivo performance and safety profiles are scarce. A novel NIR‐II fluorophore, XW‐03‐66, with a fluorescence quantum yield (QY) of 6.0% in aqueous media is reported. XW‐03‐66 self‐assembles into nanoparticles (≈80 nm) and has a systemic circulation half‐life (t 1/2) of 11.3 h. In mouse models of human papillomavirus (HPV)+ and HPV‐ OSCC, XW‐03‐66 outperformed indocyanine green (ICG), a clinically available NIR dye, and enabled intraoperative NIR‐II image‐guided resection of the tumor and adjacent draining lymph node with negative margins. In vitro and in vivo toxicity assessments revealed minimal safety concerns for in vivo applications.

Keywords

Mice, Animals, Humans, Papillomavirus Infections, Spectroscopy, Near-Infrared, Indocyanine Green, Fluorescent Dyes, Mouth Neoplasms, intraoperative imaging, mesoscopic solute‐rich clusters, NIR‐II margin delineation, NIR‐II probe, organic nanofluorophore, self‐assembling

Published Open-Access

yes

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