Primary Mouse Choroidal Endothelial Cell Culture

Authors

Qiuhua Yang
Yongfeng Cai
Qian Ma
Yuqing Huo

Language

English

Publication Date

6-20-2025

Journal

Bio-protocol Journal

DOI

10.21769/BioProtoc.5355

PMID

40620805

PMCID

PMC12222637

PubMedCentral® Posted Date

6-20-2025

PubMedCentral® Full Text Version

Post-print

Abstract

The study of choroidal endothelial cells is essential for understanding the pathological mechanisms underlying choroidal neovascularization and other vision-threatening disorders. Traditional methods for isolating and culturing primary endothelial cells often yield mixed populations or require specialized equipment, limiting their widespread use. Here, we present a straightforward protocol for isolating and culturing primary mouse choroidal endothelial cells. This protocol involves enzymatic digestion of choroidal tissue, magnetic-activated cell sorting (MACS) to enrich CD31+ endothelial cells, and optimized culture conditions to promote cell proliferation and maintain endothelial phenotype. The protocol is strategic, reproducible, and requires minimal specialized equipment, making it accessible for researchers across various fields. By providing a robust method for obtaining pure choroidal endothelial cell cultures, this protocol facilitates the study of cell-specific behaviors and responses, advancing research into choroidal vascular diseases.

Keywords

Mouse choroidal endothelial cells, RPE/choroid complex, Matrigel explant culture, CD31+ cell enrichment, Endothelial-to-mesenchymal transition (EndMT)

Published Open-Access

yes

Recommended Citation

Yang, Qiuhua; Cai, Yongfeng; Ma, Qian; et al., "Primary Mouse Choroidal Endothelial Cell Culture" (2025). Faculty, Staff and Students Publications. 6457.
https://digitalcommons.library.tmc.edu/baylor_docs/6457