The Greenbeard Gene tgrB1 Regulates Altruism and Cheating in Dictyostelium discoideum

Authors

Mariko Katoh-Kurasawa
Peter Lehmann
Gad Shaulsky

Publication Date

5-11-2024

Journal

Nature Communications

DOI

10.1038/s41467-024-48380-4

PMID

38734736

PMCID

PMC11088635

PubMedCentral® Posted Date

5-11-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Altruism, Chemotaxis, Dictyostelium, Membrane Proteins, Mutation, Protozoan Proteins, Signal Transduction, Spores, Protozoan, Genetics, Developmental biology, Evolutionary developmental biology

Abstract

Greenbeard genetic elements encode rare perceptible signals, signal recognition ability, and altruism towards others that display the same signal. Putative greenbeards have been described in various organisms but direct evidence for all the properties in one system is scarce. The tgrB1-tgrC1 allorecognition system of Dictyostelium discoideum encodes two polymorphic membrane proteins which protect cells from chimerism-associated perils. During development, TgrC1 functions as a ligand-signal and TgrB1 as its receptor, but evidence for altruism has been indirect. Here, we show that mixing wild-type and activated tgrB1 cells increases wild-type spore production and relegates the mutants to the altruistic stalk, whereas mixing wild-type and tgrB1-null cells increases mutant spore production and wild-type stalk production. The tgrB1-null cells cheat only on partners that carry the same tgrC1-allotype. Therefore, TgrB1 activation confers altruism whereas TgrB1 inactivation causes allotype-specific cheating, supporting the greenbeard concept and providing insight into the relationship between allorecognition, altruism, and exploitation.

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